Biological properties of a Haemophilus influenzae restriction enzyme, Hind I
نویسندگان
چکیده
منابع مشابه
Restriction enzyme analysis of plasmids from Haemophilus influenzae.
Examination of Haemophilus influenzae strains isolated in New Orleans revealed ampicillin-resistant strains with plasmids of size classes not previously detected in North America. The molecular weight of plasmids in five ampicillin-resistant strains ranged from 0.8 x 10(6) daltons (0.8 Mdal) to 36 Mdal. The molecular weights of the plasmids were determined by sucrose gradient centrifugation, el...
متن کاملA restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
The major breakthrough in molecular biology that permitted the “cutting and pasting” of DNA fragments was the discovery by Smith and Wilcox of a restriction enzyme (they called it endonuclease R) from Hemophilus influenzae (now called Haemophilus influenzae). These investigators reported the isolation of an enzyme that was capable of cleaving double-stranded native T7 phage DNA and other foreig...
متن کاملHaemophilus Influenzae B
یکی از شایع ترین مننژیت باکتریایی در شیرخواران، هموفیلوس آنفلوانزا از نوع B (HIB) می باشد. به منظور تهیه و عرضه واکسنی جهت حفظ کودکان در مقابل این بیماری به بازار جهانی، تحقیقاتی انجام و نوعی واکسن در بخش تحقیقاتی آکسفورد مورد آزمایش و ارزیابی قرار گرفت تا در صورت تمایل والدین و کارکنان بهداشتی، تجویز همزمان آن با واکسن سه گانه و فلج اطفال عملی گردد.
متن کاملVaccine Candidates against Nontypeable Haemophilus influenzae: a Review
Nonencapsulated, nontypeable Hemophilus influenzae (NTHi) remains an important cause of acute otitis and respiratory diseases in children and adults. NTHi bacteria are one of the major causes of respiratory tract infections, including acute otitis media, cystic fibrosis, and community-acquired pneumonia among children, especially in developing countries. The bacteria can also cause chronic dise...
متن کاملApplication of multilocus enzyme gel electrophoresis to Haemophilus influenzae.
Multilocus enzyme electrophoresis was adapted to the study of Haemophilus influenzae. Protein extracts from sonicated whole bacteria were subjected to starch gel electrophoresis. After staining with substrates, the position of each isoenzyme (electromorph) was registered. Each isolate was assigned an electrophoretic type (ET) by the combination of electromorphs for the enzymes stained. Twenty-s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1976
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.127.2.848-854.1976